diff --git a/host/lib/CMakeLists.txt b/host/lib/CMakeLists.txt
index 2fd98cb0f..7debff32f 100644
--- a/host/lib/CMakeLists.txt
+++ b/host/lib/CMakeLists.txt
@@ -65,7 +65,26 @@ LIBUHD_REGISTER_COMPONENT("USB" ENABLE_USB ON "ENABLE_LIBUHD;LIBUSB_FOUND" OFF O
 # Devices
 LIBUHD_REGISTER_COMPONENT("B100" ENABLE_B100 ON "ENABLE_LIBUHD;ENABLE_USB" OFF OFF)
 LIBUHD_REGISTER_COMPONENT("B200" ENABLE_B200 ON "ENABLE_LIBUHD;ENABLE_USB" OFF OFF)
-LIBUHD_REGISTER_COMPONENT("B300" ENABLE_B300 ON "ENABLE_LIBUHD" OFF OFF)
+# B300 support is available on Linux and Windows.
+# Only Linux requires the nib310rio kernel driver userspace header.
+if(CMAKE_SYSTEM_NAME STREQUAL "Linux")
+    find_file(NIB310RIO_HEADER
+        NAMES nib310rio/nib310rio.h
+        DOC "nib310rio kernel driver userspace header (from nib310rio-dev package)")
+    if(NIB310RIO_HEADER)
+        set(NIB310RIO_FOUND TRUE)
+    else()
+        set(NIB310RIO_FOUND FALSE)
+        message(STATUS "nib310rio/nib310rio.h not found — B300 support requires the nib310rio-dev package.")
+    endif()
+elseif(WIN32)
+    # Windows B300 support does not depend on nib310rio/nib310rio.h.
+    set(NIB310RIO_FOUND TRUE)
+else()
+    # B300 is not supported on other platforms (e.g., macOS).
+    set(NIB310RIO_FOUND FALSE)
+endif()
+LIBUHD_REGISTER_COMPONENT("B300" ENABLE_B300 ON "ENABLE_LIBUHD;NIB310RIO_FOUND" OFF OFF)
 LIBUHD_REGISTER_COMPONENT("USRP1" ENABLE_USRP1 ON "ENABLE_LIBUHD;ENABLE_USB" OFF OFF)
 LIBUHD_REGISTER_COMPONENT("USRP2" ENABLE_USRP2 ON "ENABLE_LIBUHD" OFF OFF)
 LIBUHD_REGISTER_COMPONENT("X300" ENABLE_X300 ON "ENABLE_LIBUHD" OFF OFF)
diff --git a/host/lib/usrp/b300/b300_pcie_session_linux.cpp b/host/lib/usrp/b300/b300_pcie_session_linux.cpp
index d439b6e4b..f24695cd5 100644
--- a/host/lib/usrp/b300/b300_pcie_session_linux.cpp
+++ b/host/lib/usrp/b300/b300_pcie_session_linux.cpp
@@ -7,7 +7,6 @@
 #include "./inchworm/user/libnifpga-usrp/src/Exception.h"
 #include "./inchworm/user/libnifpga-usrp/src/SysfsFile.h"
 #include "./inchworm/user/libnifpga-usrp/src/Timer.h"
-#include "./inchworm/user/libnifpga-usrp/src/nib310rio.h"
 #include "b300_regs.hpp"
 #include <uhd/config.h>
 #include <uhd/exception.hpp>
@@ -15,6 +14,7 @@
 #include <uhdlib/usrp/b300/b300_pcie_fifo.hpp>
 #include <uhdlib/usrp/b300/b300_pcie_session.hpp>
 #include <fcntl.h>
+#include <nib310rio/nib310rio.h>
 #include <system_error>
 #include <unistd.h>
 #include <cerrno>
diff --git a/host/lib/usrp/b300/inchworm/user/libnifpga-usrp/src/Fifo.cpp b/host/lib/usrp/b300/inchworm/user/libnifpga-usrp/src/Fifo.cpp
index 79e7c0fcf..ba3d45e4b 100644
--- a/host/lib/usrp/b300/inchworm/user/libnifpga-usrp/src/Fifo.cpp
+++ b/host/lib/usrp/b300/inchworm/user/libnifpga-usrp/src/Fifo.cpp
@@ -15,7 +15,7 @@
 #include "Fifo.h"
 #include "ErrnoMap.h"
 #include "Exception.h"
-#include "nib310rio.h"
+#include <nib310rio/nib310rio.h>
 #include <unistd.h> // sysconf
 #include <cassert> // assert
 #include <cstdlib> // valloc
diff --git a/host/lib/usrp/b300/inchworm/user/libnifpga-usrp/src/nib310rio.h b/host/lib/usrp/b300/inchworm/user/libnifpga-usrp/src/nib310rio.h
deleted file mode 100644
index 0c87279a2..000000000
--- a/host/lib/usrp/b300/inchworm/user/libnifpga-usrp/src/nib310rio.h
+++ /dev/null
@@ -1,68 +0,0 @@
-/* SPDX-License-Identifier: GPL-2.0+ WITH Linux-syscall-note */
-#ifndef __NIB310RIO_H__
-#define __NIB310RIO_H__
-
-#include <linux/types.h>
-
-#define NIB310RIO_IOC_MAGIC (93)
-
-struct ioctl_nib310rio_array
-{
-    __u32 offset;
-    __u32 count;
-    __u32 data[0];
-};
-
-struct ioctl_nib310rio_fifo_set_buf
-{
-    __u32 fd;
-};
-
-struct ioctl_nib310rio_fifo_acquire
-{
-    __aligned_u64 elements;
-    __aligned_u64 available;
-    __u32 timeout_ms;
-    __u32 timed_out;
-};
-
-struct ioctl_nib310rio_irq_wait
-{
-    __u32 ctx;
-    __u32 mask;
-    __u32 timeout_ms;
-    __u32 asserted;
-    __u32 timed_out;
-};
-
-struct ioctl_nib310rio_reg32
-{
-    __u32 offset;
-    __u32 value;
-};
-
-struct ioctl_nib310rio_reg64
-{
-    __u32 offset;
-    __u64 value;
-};
-
-#define NIB310RIO_IOC_FIFO_STOP  _IO(NIB310RIO_IOC_MAGIC, 2)
-#define NIB310RIO_IOC_FIFO_START _IO(NIB310RIO_IOC_MAGIC, 3)
-#define NIB310RIO_IOC_FIFO_SET_BUF \
-    _IOW(NIB310RIO_IOC_MAGIC, 4, struct ioctl_nib310rio_fifo_set_buf)
-#define NIB310RIO_IOC_FIFO_ACQUIRE \
-    _IOWR(NIB310RIO_IOC_MAGIC, 5, struct ioctl_nib310rio_fifo_acquire)
-#define NIB310RIO_IOC_FIFO_RELEASE   _IOW(NIB310RIO_IOC_MAGIC, 6, __u64)
-#define NIB310RIO_IOC_FIFO_GET_AVAIL _IOR(NIB310RIO_IOC_MAGIC, 7, __u64)
-#define NIB310RIO_IOC_IRQ_CTX_ALLOC  _IOR(NIB310RIO_IOC_MAGIC, 10, __u32)
-#define NIB310RIO_IOC_IRQ_CTX_FREE   _IOW(NIB310RIO_IOC_MAGIC, 11, __u32)
-#define NIB310RIO_IOC_IRQ_WAIT \
-    _IOWR(NIB310RIO_IOC_MAGIC, 12, struct ioctl_nib310rio_irq_wait)
-#define NIB310RIO_IOC_IRQ_ACK _IOW(NIB310RIO_IOC_MAGIC, 13, __u32)
-#define NIB310RIO_IOC_PEEK32  _IOWR(NIB310RIO_IOC_MAGIC, 20, struct ioctl_nib310rio_reg32)
-#define NIB310RIO_IOC_POKE32  _IOW(NIB310RIO_IOC_MAGIC, 21, struct ioctl_nib310rio_reg32)
-#define NIB310RIO_IOC_PEEK64  _IOWR(NIB310RIO_IOC_MAGIC, 22, struct ioctl_nib310rio_reg64)
-#define NIB310RIO_IOC_POKE64  _IOW(NIB310RIO_IOC_MAGIC, 23, struct ioctl_nib310rio_reg64)
-
-#endif